Bactericidal treatment of hatching eggs. III. Effect of organic contaminants on efficacy of egg sanitizers
2001
Bailey, J.S. | Cox, N.A. | Berrang, M.E.
The effectiveness of three sanitizers in killing Salmonella during room temperature storage with and without contaminating feces, egg, or chicken rinse for 48 h was tested. Uncontaminated sanitizers were tested, as were 50-mL solutions of 0.035% polyhexamethylenebiguanide hydrochloride (PHMB), 0.39% (1:256) Tektrol, and 1.4% H2O2, which were contaminated with 10 mL of a feces slurry, 10 mL of mixed egg, or 10 mL of chicken rinse. Each container was inoculated with approximately 1,000 cells of S. typhimurium and cultured after 1 and 5 min. After 24 and 48 h of storage, each original container was reinoculated and assayed for the new inoculum at 1 and 5 min postinoculation. Complete eradication of Salmonella was noted after 1 or 5 min exposure to fresh or stored uncontaminated sanitizer solutions. Tektrol became less active against Salmonella when contaminated with egg and stored for 24 h or more. However, feces or chicken rinse did not interfere with the activity of Tektrol. Hydrogen peroxide deteriorated most with storage with chicken feces and to a lesser extent chicken rinse. PHMB lost efficacy when exposed to chicken feces for 24 h or more, but was not affected by the other contaminants.
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