Genetic variation of isolated Picea balfouriana populations from the southeast of the Qinghai-Tibet Plateau | Variation génétique des populations isolées de Picea balfouriana du sud-est du plateau Qinghai-Tibet
2009
Lu, Zhuoxuan | Wang, Yuhua | Zhang, Xiaolu | Korpelainen, Helena | Li, Chunyang
• The objective of this work is to estimate the level of genetic variation and pattern of genetic structure of isolated Picea balfouriana populations. • Nine SSR markers and six STS markers were assayed in ten natural populations of P. balfouriana, which is a regionally distributed conifer species in the southeast of the Qinghai-Tibet Plateau. • Expected heterozygosity ranged from 0.592 to 0.710 based on SSRs, and from 0.489 to 0.635 based on STS markers. The SSR and STS markers revealed that 11% and 12% of variation, respectively, was present among populations. However, the SSRs showed no deviation from the Hardy-Weinberg equilibrium (FIS= −0.030), unlike the STS markers (FIS= 0.249). In addition, assignment methods showed that individuals from the same sampling site usually cluster together. • Our results indicated that the distribution of genetic variation and population genetic structure of P. balfouriana may be attributed to habitat fragmentation and heterogeneous environments caused by the complex topographic environment in the Qinghai-Tibet Plateau. The population genetic information obtained in our study will benefit the development and utilization of appropriate conservation and breeding strategies for P. balfouriana.
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