AtJ6, a unique J-domain protein from Arabidopsis thaliana
2000
Kroczynska, B. | Coop, N.E. | Miernyk, J.A.
An Arabidopsis thaliana cDNA encoding a protein, AtJ6, related to Escherichia coli DnaJ was sequenced. DnaJ was first isolated as a heat shock protein from E. coli. Translation of the AtJ6 nucleotide sequence yields a protein with an N-terminal J-domain, but which lacks the G/F and C-rich domains characteristic of DnaJ. In addition to the J-domain, one region of the sequence has homology with the prokaryotic FtsA protein. The remainder of the AtJ6 sequence is not notably related to anything in the sequence databases. The AtJ6 sequence represents a unique member of the A. thaliana J-domain protein family. The results of Northern analysis revealed that the AtJ6 mRNA is expressed in all organs of A. thaliana; expression was high in leaves, flowers and siliques, and low in roots. The results of Southern analysis are consistent with a single unique gene. Nucleotide sequence data have been submitted to the GenBank database under the accession number AF037168.
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