NoiD, a DedA membrane protein required for homeostasis maintaining of Rhizobium leguminosarum biovar viciae during symbiosis with Pisum sativum
2022
Li, Xiaofang | Xu, Jiaming | Wei, Yajuan | Chen, Zirui
Symbiotic nitrogen fixation is based on the symbiotic relationship between rhizobia and plants and a sustainable green way to maintain fertility in modern agriculture. However, the lack of a thorough understanding on the mechanisms seriously hinders the application of this biological way to enhance agricultural production. In the present study, we discovered an interesting mutation of NoiD in Rhizobium leguminosarum biovar viciae 3841 (R.l. viciae 3841). This DedA membrane protein, affected the phenotypic development on both rhizobia and host (Pisum sativum). Compared with R.l. viciae 3841, the noiD mutant strain (△ⁿᵒⁱᴰ) was present as independent small colonies, and the proportion of Rhizobium cells that were of shorter size increased. Inoculating △ⁿᵒⁱᴰ to Pisum sativum resulted in premature senility and nodulation defects at 28 dpi with a 28.86% reduction of root nitrogen fixation efficiency compared to wild-type group. Furthermore, we showed that these are mainly attributed to an increase in temperature, pH, and ionic osmolarity sensitivity of the rhizobia with the noiD mutation. These results indicate that NoiD participates in the homeostasis mechanism of R.l. viciae 3841, and can directly influence nodulation and nitrogen fixation efficiency in Pisum sativum.
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