Comparacion de tres metodos de analisis de silicio en tejido foliar de pepino | Comparison of three methods of silicon analysis in cucumber leaf tissue
2004
Parra-Terraza, S. | Baca-Castillo, G.A. | Carrillo-Gonzalez, R. | Kohashi-Shibata, J. | Martinez-Garza, A. | Trejo-Lopez, C.
Silicon may be beneficial for plants growing under biotic or abiotic stress conditions. For this reason, its chemical analysis in plant tissue is important, from the perspective of crop nutrition. There are several analytical methods for the quantification of silicon; nevertheless, these methods have one or more disadvantages. In addition, plant standard reference material is not available, so there are some uncertainties about analytical procedures to be used for accurate silicon estimation. In this study three methods of silicon extraction and quantification were compared: (1) high-temperature fusion with NaOH (reference method); (2) wet ashing with acid solution of HNO3:H2SO4:HClO4; and (3) dry ashing with dissolution of the ash in HF. Cucumber leaves from plants grown in a greenhouse were utilized. Nine treatments of nutrient solutions resulted from the combination of two factors: SiO3(2-) concentrations (0, 0.75, and 1.5 mol m(-3)) and osmotic potential (-0.036, -0.072 and -0.108 MPa). The silicon quantified by the dry ashing and fusion methods were statistically equivalent. The regression analysis between silicon values quantified with the two methods indicated a significant linear relationship with a determination coefficient (R2) of 0.9488 and the variation coefficient (CV) of 15.59%. The method based on wet digestion overestimated the silicon content by 256.8% with respect to the fusion method. Its R2 value was 0.5599 and the CV was 45.71%.
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