Toxicity of a secondary metabolite produced by Simplicillium chinense Snef5 against the root-knot nematode Meloidogyne incognita
2020
Zhao, Di | Zhu, Xiaofeng | Chen, Lijie | Liu, Weiwei | Chen, Jingsheng | Wang, Shuo | Zang, Jian | Duan, Yuxi | Liu, Xiaoyu
We studied the bioactive compound of the fungus Simplicillium chinense strain Snef5, isolated from its culture filtrate. Following purification by chromatography, the structure of the secondary metabolite, identified as 2,6-pyridinedicarboxylic acid, was determined by spectroscopic methods (IR, ¹H, and ¹³C NMR) and scanning electron microscopy (SEM). The metabolite exhibited antimicrobial activity against M. incognita with an LD50 of 0.229 mg/ml. The light microscope was used to observe the surface and internal cellular micro-structure of the nematodes. The results of the microscopy indicated that the anatomy of the J₂ of M. incognita was disrupted following exposure to 2,6-pyridinedicarboxylic acid. This is the first report on the activity of 2,6-pyridinedicarboxylic acid of S. chinense metabolites on the root knot nematode. It showed a high level of nematicidal activity. Our data on the inhibitory effects of 2,6-pyridinedicarboxylic acid suggest that secondary metabolites from Snef5 have potential use as novel biological control agents.
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