High-performance liquid chromatography determination of erythrocyte membrane phospholipid composition in several animal species

Engen, R.L.; Clark, C.L.

High-performance liquid chromatography determination of erythrocyte membrane phospholipid composition in several animal species

1990

Engen, R.L. | Clark, C.L.

High-performance liquid chromatography (HPLC) was used to determine the phospholipid (PL) composition of ovine, equine, bovine, porcine, and canine RBC membranes. Procedural modifications of established techniques provided for separation of 7 PL within a 15- to 20-minute sample run. Significant (P < 0.05) differences were detected in RBC membrane PL composition among the various species. The concern for physiologic properties associated with hemolysis and/or sedimentation rate must include evaluation of differences in the PL bilayer structure.

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