Genetic Diversity of Potato virus Y Infecting Tobacco Crops in China
2011
Tian, Y.P. | Liu, J.L. | Zhang, C.L. | Liu, Y.Y. | Wang, B. | Li, X.-D. | Guo, Z.K. | Valkonen, J.P.T.
Genetic variability of Potato virus Y (PVY) isolates infecting potato has been characterized but little is known about genetic diversity of PVY isolates infecting tobacco crops. In this study, PVY isolates were collected from major tobacco-growing areas in China and single-lesion isolates were produced by serial inoculation on Chenopodium amaranticolor. Most isolates (88%) caused systemic veinal necrosis symptoms in tobacco. Of these, 16 isolates contained a PVYO-like coat protein (CP) and PVYN-like helper component proteinase (HC-pro) and, in this respect, were similar to the PVYN-Wi, PVYN:O, and PVY-HN2 isolates characterized from potato in Europe, the United States, and China, respectively; two isolates contained a PVYO-like HC-pro and a PVYN-like CP; another two isolates had recombination junctions in the CP-encoding region. Both the HC-pro and CP of PVY were under negative selection as a whole; however, seven amino acids in HC-pro and six amino acids in CP were under positive selection. Selection pressures differed between the subpopulations of PVY distinguished by phylogenetic analysis of HC-pro and CP sequences. When PVY isolates from potato were included, no host-specific clustering of the PVY isolates was observed in phylogenetic and nucleotide diversity analyses, suggesting frequent spread of PVY isolates between potato and tobacco crops in the field.
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