Analysis of aflatoxin B1 in corn using capillary electrophoresis with laser-induced fluorescence detection
1997
Maragos, C.M. | Greer, J.I.
A capillary electrophoresis (CE) method was developed for the quantitation of aflatoxin B1 (AFB1) in corn. The instrumentation can be assembled easily from readily available components and takes advantage of the native fluorescence of AFB1. Corn was extracted and aflatoxins were isolated using either silica column or affinity column cleanup procedures. Following cleanup, the analysis of each sample required 15 min: 10 min for the electrophoresis and 5 min for rinsing the capillary. The CE method was compared to an established HPLC method for the determination of AFB1 in corn. The limit of detection by CE was 0.5 ppb, with a useful range of 1-100 ppb of AFB1 in spiked corn. Recovery of AFB1 averaged 85% over the range of 1-50 ppb (89% by HPLC). Forty naturally contaminated corn samples examined by using both methods showed good agreement (r2 = 0.969). The reported CE method is suitable for the routine analysis of corn samples as an alternative to HPLC.
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