Potential of Bacillus sp. to produce polyhydroxybutyrate from biowaste
2009
Kumar, T. | Singh, M. | Purohit, H.J. | Kalia, V.C.
To test the Bacillus strains for their abilities to produce polyhydroxybutyrate (PHB) from different sugars and biowaste (Pea-shells). Six Bacillus strains were checked for their ability to produce PHB from GM2 medium supplemented with different sugars at the rate of 1% (w/v) and from biowaste and GM2 (BW : M) combinations (3 : 7, 1 : 1, 7 : 3). Glucose supplemented GM2 medium resulted in maximum PHB production of 435 mg l⁻¹ constituting 31-62% w/w of the total cell dry mass. Substituting GM2 medium to the extent of 50% with biowaste (pea-shell slurry) resulted in 945-1205 mg l⁻¹ PHB (55-65% w/w). Optimization for additional nitrogen supplementation, inoculum size resulted in a final PHB production of 3010-3370 mg l⁻¹ equivalent to 300 g kg⁻¹ biowaste (dry wt). The Bacillus strains were able to produce PHB from biowaste (Pea-shells) as cheap source of substrate. This is the first report on usage of pea-shells as feed for PHB production, opening new possibilities for its use for production of PHB and Bacillus as potential candidate for the purpose.
显示更多 [+] 显示较少 [-]