A highly specific one-step PCR-assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis
2001
Arnold T. | Hensel, A. | Hagen, R. | Aleksic, S. | Neubauer, H. | Scholz, H.C.
Based on differences within the yopT-coding region of Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis, a rapid and sensitive one-step polymerase chain reaction assay with high specificity for pathogenic Y. enterocolitica was developed. By this method pathogenic isolates of Y. enterocolitica can be easily identified and discriminated from other members of this genus. The entire coding sequence of the yopT effector gene of Y. pseudotuberculosis Y36 was determined.
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书目信息
Systematic and applied microbiology
卷
24
期
2
页码
285
- 289
ISSN
0723-2020
其它主题
Rapid methods; Base sequence; Differentiation; Sensitivity and specificity; Time factors; Cytotoxins; Virulence genes; Yopt gene; Yersinia infections; Cysteine endopeptidases; Molecular sequence data
语言
英语
类型
Journal Article; Text
2024-02-28
MODS