Determination of tetramethylthiuram disulfide (thiram) for residual analysis in food using spectrophotometry coupled with a solid-phase reactor (SPR) in a flow system
2014
Silva Junior, Ranulfo C. | Kushikawa, Ricardo T. | Gomes, Homero M. | Teixeira, Marcos F. S.
An analytical method was developed for quantification of the fungicide thiram (tetramethylthiuram disulfide), based on its capacity to complex Cu ²⁺ ions. A solid-phase reactor (SPR) was coupled in a flow system containing a spectrophotometer as detector. The SPR consisted of a tube filled with finely ground resin, in which copper(II) phosphate was immobilised prior to polymerisation. Contact of thiram with the Cu ²⁺ ions immobilised in the resin resulted in the formation of a strongly coloured Cu ²⁺/thiram complex. Maximum absorbance of the Cu ²⁺/thiram complex was obtained at a wavelength of 439 nm. The usefulness of the methodology has been evidenced by the determination of thiram residues in corn and bean grains. Previously, a variety of transition metal cations which would form coloured complexes with tetramethylthiuram disulfide were tested.
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