Production and optimization of biohydrogen from saccharolytic actinobacterium, Streptomyces rubiginosus (SM16), using sugarcane molasses
2017
Sivarajan, Anbalmani | Shanmugasundaram, Thangavel | Thirumalairaj, Jeyaraman | Balagurunathan, Ramasamy
The Actinobacteria are known to produce secondary metabolites like antibiotics, enzymes and pigments and also used in bioremediation. Recently the Actinobacteria have also been reported as a source of biofuels too. Here, the hydrogen production was carried out at different concentrations of sugarcane molasses, pH and temperature through dark H₂ fermentation using the actinobacterium Streptomyces rubiginosus (SM16) isolated from a sugarcane molasses dumping site. The maximum biohydrogen production rate was found to be 296 ± 0.042 mL/L at 4% sugarcane molasses. At the optimized pH of 7.0, the maximum biohydrogen production rate was 283 ± 0.015mL/L, whereas at the optimized temperature of 30°C, the yield of biohydrogen was 266 ± 0.022 mL/L using the effective inoculum SM16. The maximum hydrogen production (407.33 ± 5.03 mL/L) was recorded in the optimized fermentation medium with sugarcane molasses 4%, pH 7.0 and temperature 30°C. Gas chromatography (GC) and high performance liquid chromatography (HPLC) analysis proved the presence of organic acid (tartaric acid – 35.7%) and other volatile fatty acids along with H₂ gas. Hence, the actinobacterium Streptomyces rubiginosus (SM16) is considered a promising source for biohydrogen production.
显示更多 [+] 显示较少 [-]