Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR
2016
Alanio, Alexandre | Sturny-Leclère, Aude | Benabou, Marion | Guigue, Nicolas | Bretagne, Stéphane
Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.
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书目信息
Journal of microbiological methods
卷
127
页码
160
- 163
ISSN
0167-7012
出版者
Elsevier B.V.
其它主题
Droplet digital pcr; Genome; Real-time quantitative pcr; Copy number variation
语言
英语
类型
Journal Article; Text
2024-02-28
MODS