Preparation and isolation of the partially hydrolyzed moiety of fumonisin B1
1995
Sydenham, E.W. | Thiel, P.G. | Shephard, G.S. | Koch, K.R. | Hutton, T.
The natural occurrence in corn of carcinogenic mycotoxins, the fumonisins, has prompted the development of potential decontamination procedures. Chemical treatment of fumonisin B1 (FB1)-contaminated corn with calcium hydroxide [Ca(OH)2] results in the base hydrolysis of FB1 (the major naturally occurring fumonisin analogue) to yield its corresponding aminopentol (AP1) and tricarballylic acid (TCA) moieties. Complete hydrolysis proceeds in a sequential reaction involving the removal of one TCA group and the formation of a partially hydrolyzed moiety (PH1), which exists as an equilibrium mixture of the two possible monoesters. PH1 was prepared by the treatment of Fusarium moniliforme culture material with Ca(OH)2 and subsequently isolated and purified using chromatographic methods. PH1 was also prepared, using similar methods, from pure FB1. The identity of the PH1 moiety was determined by liquid chromatography-electrospray mass spectrometry.
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