Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA

Wawrzyniak-Turek, Katarzyna; Höbartner, Claudia

Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA

2014

Wawrzyniak-Turek, Katarzyna | Höbartner, Claudia

We describe a general and simple method to identify catalytically and structurally important nucleotides in functional RNAs. Our approach is based on statistical replacement of each nucleoside with a non-nucleosidic spacer (C3 linker, Δ), followed by separation of active library variants and readout of interference effects by analysis of enzymatic primer extension reactions.

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