Discovery of genes associated with cadmium accumulation from gill of scallop Chlamys farreri based on high-throughput sequencing
2016
Zhang, Hui | Zhai, Yuxiu | Yao, Lin | Jiang, Yanhua | Li, Fengling
Chlamys farreri is an important economic mollusk and is able to accumulate cadmium (Cd) excessively. Two cDNA libraries from gill of C. farreri which were Cd treated or not were generated based on the Illumina sequencing platform in order to identify differentially expressed genes (DEGs) associated with cadmium accumulation. The analysis of comparative transcriptomics identified 82,800 unigenes and 128 differentially expressed genes. Of these, 8 of the most significant DEGs were verified by real-time PCR, showing consistent trends in expression patterns between the two techniques. KEGG pathways analysis revealed that 37 associated processes were highly enriched. A set of key DEGs were identified that may play important roles in cadmium exposure. Our work presents an overview of gene expression change during cadmium exposure to C. farreri and identifies a series of candidate genes and pathways for further research on the molecular mechanisms of cadmium accumulation.
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