Induction of systemic resistance in Lycopersicon esculentum cv. PKM1 (tomato) against Cucumber mosaic virus by UV-B irradiation on calli
2011
Sudhakar, N. | Murugesan, K.
Studies were done to evaluate the effect of UV-B irradiation on calli to induce resistance in the regenerated Lycopersicon esculentum cv. PKM1 (tomato) plantlets against Cucumber mosaic virus (CMV)-induced diseases. Plantlets regenerated from the UV-B (TL, TM, TH) treated calli are referred to as TL, TM and TH plantlets respectively, and they hold remarkably increased soluble phenolics, salicylic acid and peroxidase content compared to control plantlets without any treatment. All the plantlets challenge-inoculated with CMV showed disease severity ranging from TM = 32%, TH = 56%, TL = 78% and C = 92%. But in contrast, ELISA results show that CMV accumulation in young uninoculated leaves were significantly less in the TH plantlets than TM plantlets, which reveals that TH treatment affects virus accumulation and also affected the plantlet's physiological properties and chlorophyll content due to high UV-B dosage. However, in TM plantlets, physiological properties were not affected and these plantlets were effectively elicited against CMV attack. Exposure of UV-B (TM) on calli certainly activates an effective defense response and empowers the tomato plantlet's survival against viral attack.
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