Conditions of transformation and regeneration of 'Induka' and 'Elista' strawberry plants
2004
Gruchala, A. | Korbin, M. | Zurawicz, E.
Efficiency of plants' transformation depends on many factors. The genotype, applied techniques and conditions of plant's modification and modified plant regeneration are the most important among them. In our studies regeneration and transformation conditions for two strawberry cultivars were determined and compared. Plants were transformed by Agrobacterium tumefaciens LBA4404 strain containing plasmid pBIN19 with nptII and gus-reporter genes. Experiment was carried out on more than 1300 leaf explants from each cultivar. Generally, 'Induka' plants characterized with higher regeneration potential than 'Elista'. The highest number of regenerated shoots was obtained on MS medium with 0.4 mg l(-1) IBA and 1.8 mg l(-1) BA (3.5 and 1.8 shoots/explant for 'Induka' and 'Elista', respectively). After plant transformation number of regenerated, transgenic shoots was higher for 'Elista' (on the average: 8.3 shoots/100 explants). The number of transgenic 'Induka' shoots, obtained at the same conditions, was twice lower (approximately 4.2). Simultaneously 'Induka' plants needed higher kanamycin concentration for transgenic explants selection than 'Elista' (25 mg l(-1)). Preliminary incubation of A. tumefaciens in LB or MS medium with acetosyringone and IAA resulted in increasing transgenic shoots number (per 100 explants: 'Induka' 4.5, 'Elista' 8.0-9.5 shoots). After using untreated bacteria for plants' transformation, number of transgenic plants varied (dependently on cultivar) from 3.8 to 7.0/100 explants. Applying LB or MS as basic medium as well as adding tobacco plant extract to these media did not significantly influence transformation efficiency.
显示更多 [+] 显示较少 [-]