Application of Bacillus alkaline protease for dehairing of buffalo skin and its histological studies
2008
Jaswal, R.K. | Kocher, G.S. | Banga, H.S. | Brar, R.S.
Alkaline proteases are eco-friendly enzymes that can be used as an alternate to chemical processes of pretanning operations in tanning industry. The alkaline proteases are known to be produced by a number of bacteria and fungi. However, industry is still in search of efficient protease producing microorganisms. In the present study, among the four potential alkaline protease producing bacterial isolates enriched from different sources, RJ25 enriched from corpses was selected on the basis of plate clearing zone method. This isolate was identified as a strain of Bacillus sp. on the basis of different morphological, biochemical and physiological tests. The protease was produced under standard conditions and used for the treatment of buffalo skin pieces (1.5"X 1.5") in crude protease of Bacillus sp. RJ 25 and 10% v/v enzyme incubation for 3 hours was found to be the optimum. The ease of plucking was used for qualitative analysis that revealed easy removal of skin hairs. The results were compared with histological and microscopic analysis of enzyme treated skin.
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