A fluorescent lectin test for mold in raw tomato juice
2000
Potts, S.J. | Slaughter, D.C. | Thompson, J.F.
Fungal (mold) contamination is an important indicator of low quality raw product in the processing tomato industry. A quantitative lectin assay was developed that was less expensive, faster, and more precise than the industry standard Howard mold count. This assay, based on a fluorescent-labeled lectin isolated from wheat germ, had a selective affinity for the chitin in fungal cell walls. Assay values were correlated with mold contamination for 4 fungal species: Alternaria alternata (r2 = 0.91), Cladosporium herbarum (r2 = 0.75), Fusarium oxysporum (r2 = 0.97), and Stemphylium botryosum (r2 = 0.99). Combining all 4 species, the lectin assay had a strong correlation (r2 = 0.76) with a linearized Howard mold count.
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