Tenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia
2020
Podosokorskaya, Olga A. | Kochetkova, Tatiana V. | Novikov, Andrei A. | Toshchakov, Stepan V. | Elcheninov, Alexander G. | Kublanov, Ilya V.
A novel anaerobic moderately thermophilic bacterium, designated strain 38H-strᵀ, was isolated from a 12m deep hot spring of the Kunashir Island shore. Gram-negative cells were non-spore-forming, motile, straight or curved filamentous rods, occasionally forming loops and knots. The strain grew at 20–65°C and pH range of 4.0–9.0 with an optimum at 50°C and pH 6.5–7.0. Strain 38H-strᵀ required 0.5–2.5% NaCl (1.5% is an optimum) for growth. It was a chemoorganoheterotroph, growing on carbohydrates (starch, pullulan, alginate, laminarin, beta-glucan) or peptide mixtures and proteins (peptone, tryptone, gelatin, and α- or β- keratins). Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso- and anteiso-C₁₅:₀. Phosphatidylethanolamine, an unidentified phospholipid, and three unidentified polar lipids were detected in cellular lipids fractions. The quinone was MK-7. The size of complete genome of strain 38H-strᵀ was 3.2 Mb; DNA G+C content was 38.3mol%. According to 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 38H-strᵀ represented a deeply branched lineage near the root of the class Bacteroidia. Based on phylogenetic analysis and phenotypic features the novel isolate was assigned to a novel family within the order Bacteroidales for which the name Tenuifilaceae fam. nov. is proposed. Strain 38H-strᵀ (=DSM 100343ᵀ =VKM B-2964ᵀ) represents the first genus and species Tenuifilum thalassicum gen. nov., sp. nov.
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