Pyrenochaeta terrestris microsclerotia production and pigmentation on onion roots
1992
Biles, C.L. | Holland, M. | Ulloa-Godinez, M. | Clason, D. | Corgan, J.
Experiments were conducted to determine variation of in vitro microsclerotia production, pigmentation, and growth of five Pyrenochaeta terrestris (Hans.) Gorenz, Walker, and Larson isolates from Texas and New Mexico. Isolates of P. terrestris, the causal agent of pink root rot, were placed on agar with 10-mm-long sections of sterile onion (Allium cepa L.) roots. Microsclerotia were present after 20 days at 20, 25, 27, or 32 C, with the number of microsclerotia dependent on the isolate. Microsclerotia were absent at 35 C. One isolate produced few microsclerotia at all temperatures. Optimum temperatures for growth on potato dextrose agar for the isolates tested were 25 and 27 C. Pigment production in roots on agar varied depending on isolate and temperature. Three isolates produced high levels of pigment in onion roots at 15, 20, 25, and 27 C. A New Mexico isolate produced significantly less visible pigment than the other New Mexico and Texas isolates. One isolate produced very little pigment at all temperatures tested. Constant fluorescent light stimulated pycnidia production in one isolate and reduced microsclerotia production in all other isolates. Isolates varied significantly in microsclerotia production and pigment synthesis.
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