Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs

Zubrzycki, Igor Z.; Klump, Horst H.

Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs

2014

Zubrzycki, Igor Z. | Klump, Horst H.

High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. teperature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a charactersitic mark on the melting profile of its parental plasmid

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