Isolation and characterization of 11 microsatellite loci from the black fly, Simulium negativum (Diptera: Simuliidae)

SPIRONELLO, MIKE; RIZVI, LEENA; CURRIE, DOUGLAS C.; DAVY, CHRISTINA M.; Murphy, Robert W.

Isolation and characterization of 11 microsatellite loci from the black fly, Simulium negativum (Diptera: Simuliidae)

2009

SPIRONELLO, MIKE | RIZVI, LEENA | CURRIE, DOUGLAS C. | DAVY, CHRISTINA M. | Murphy, Robert W.

Eleven polymorphic microsatellite loci were isolated and developed from the black fly, Simulium negativum, a member of the Simulium arcticum sibling species complex. The observed heterozygosity of the 11 loci ranged from 0.03 to 0.83. The number of alleles per locus ranged from 8 to 19. Significant linkage disequilibria were encountered only for the primer pairs BF7-1 with BF7-5 and BF6-32 with BF7-16. Presumably, these microsatellite loci can be used to study genetic structure within the entire S. arcticum complex.

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