Effect of dietary restriction during gestation on amino acid compositon and myofibrillar and collagen contents of skeletal muscle in gilts mated at puberty
1992
Zarkadas, C.G. | Larmond, E. | Elliot, J.I. | Khalili, A.D. | Beddard-Neil, C.
Sixteen Yorkshire gilts, mated at puberty, were used to study the effect of severe dietary restriction on the skeletal muscle amino acid composition, and its myofibrillar and connective tissue protein contents, and to determine their potential use as indices for evaluating carcass quality. The proposed chemical approach is based on the direct determination of the myofibrillar myosin and actin contents of skeletal muscle from the amounts of Ntau-methylhistidine found in their 96-h acid hydrolysates. Collagen and collagen-like proteins can be determined from the amounts of 5-hydroxylysine present. The semimembranosus muscle contained 10.9-12.4% actin and 22.8-23.9% myosin, corresponding to 21.0 and 44.0% of the myofibrillar proteins (51.9-54.3% of the total muscle proteins). The total skeletal muscle collagen ranged from 4.8 to 5.9% in the semimembranosus of once-mated gilts compared to 3.7-4.2% in the unmated controls. In all cases the increased muscle collagen appeared to be associated with changes during gestation, and meat from gilts that had farrowed tended to be less tender than that from controls.
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