Aminocella lysinolytica gen. nov., sp. nov., a L-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms
2015
Ueki, Atsuko | Shibuya, Toru | Kaku, Nobuo | Ueki, Katsuji
A strictly anaerobic bacterial strain (WN037ᵀ) was isolated from a methanogenic reactor. Cells were Gram-positive rods. Strain WN037ᵀwas asaccharolytic. The strain fermented L-lysine in the presence of B-vitamin mixture or vitamin B₁₂and produced acetate and butyrate. L-arginine and casamino acids poorly supported the growth. Strain WN037ᵀused neither other amino acids nor organic acids examined. The strain had C₁₈:₁ω7c, C₁₆:₀and C₁₈:₁ω7c DMA as the predominant cellular fatty acids. The genomic DNA G + C content was 44.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN037ᵀin the family Eubacteriaceae in the class Clostridia. The closest relative was Eubacterium pyruvativorans (sequence similarity, 92.8 %). Based on the comprehensive analyses, the novel genus and species, Aminocella lysinolytica gen. nov., sp. nov. was proposed to accommodate the strain. The type strain is WN037ᵀ(= JCM 19863ᵀ = DSM 28287ᵀ).
显示更多 [+] 显示较少 [-]