A Phosphate-Accumulating Microbial Community in the Laboratory Bioreactor Predominated by “Candidatus Accumulibacter”
2022
Pelevina, A. V. | Berestovskaya, Yu. Yu | Grachev, V. A. | Dorofeev, A. G. | Slatinskaya, O. V. | Maksimov, G. V. | Kallistova, A. Yu | Nikolaev, Yu. A. | Grouzdev, E. V. | Ravin, N. V. | Pimenov, N. V. | Mardanov, A. V.
A microbial community enriched with phosphate-accumulating organisms (PAO) was obtained in a laboratory sequencing batch reactor after 150 days of cultivation. Analysis of phosphate dynamics in the medium and the results of Raman scattering spectroscopy indicated the cycle of phosphate consumption and release during cultivation under oxic and anoxic conditions, respectively, which was typical of PAO. The highest content of intracellular phosphorus was 16.5 ± 0.15% of the dry ash-free biomass. Molecular genetic analysis and FISH revealed the taxonomic diversity of the microbial community, in which members of the “Ca. Аccumulibacter” group were the major PAO.
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