Comparison of Purine Metabolism in Suspension Cultured Cells of Different Growth Phases and Stem Tissue of Catharanthus roseus
2014
Hirose, Fumiko | Ashihara, Hiroshi
The overall metabolism of [8-¹⁴C]adenine, [8-¹⁴C]adenosine, [8-¹⁴C]guanine, [8-¹⁴C]hypoxan- thine and [8-¹⁴C]inosine in suspension cultured cells of the lag, cell division, cell expansion and stationary phases and stem tissue of Catharanthus roseus was studied. On a fresh weight basis, absorption of all purine bases and nucleotides by the cultured cells was 10-400 times higher than that by the stem tissue. The conventional pathways of purine salvage and degradation were operative both in the cultured cells and stem tissue. [8-¹⁴C]Adenine and [8-¹⁴C]adenosine were extensively salvaged to nucleotides and nucleic acids in both the cultured cells and the stem tissue. [8-¹⁴C]-Guanine, [8-¹⁴C]hypoxanthine and [8-¹⁴C]inosine were incorporated into either salvage products (nucleotides and nucleic acids) or degradation products (CO₂, allantoin and allantoic acid) of cultured cells and stem tissue. The highest incorporation rate expressed as a percentage of absorbed ¹⁴C into the degradation products was observed in the lag phase (12h-old) cultured cells. Incorporation of all precursors into nucleotides and bases was much higher in the stem tissue than in the culltured cells.
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