The effects of two preservatives on peroxidase activity and total protein content in Strelitzia reginae during cold storage
2016
Vieira, Marcos R. da S. | Pessoa, Luiz G. M. | Júnior, Walter S. E. | Leal, Ygor H. | da Silva, Luzia F. | Silva, Rafael C. | dos Santos, Eduardo S. | Amorim, Thialla L. | de Medeiros, Damina C.
This study aimed to assess changes in peroxidase activity and total protein content of cut flowers of Strelitzia reginae that were treated with the chemical preservatives 8-hydroxyquinoline citrate (8-HQC) or chlorine during cold storage. The bases of the cut flowers were immersed in 1.5 L of tap water with 48 h pulsing treatments (a control with no chemical preservatives (0 mg·L⁻¹, 100 mg·L⁻¹ chlorine, 250 mg·L⁻¹ 8-HQC or 500 mg·L⁻¹ 8-HQC) during cold storage at 10.5°C and 90% relative humidity. The solutions were then replaced with tap water and the cut flowers remained in cold storage for 12 days. For biochemical characterization of peroxidase activity and total protein content, tissue samples (sepals, petals, stems, and bracts) were collected and analyzed at 0, 4, 8, and 12 days after treatment. There was an increase in peroxidase activity in petals, stems, and bracts and a decrease in total protein content, regardless of the treatment used. In this experiment, therefore, differences in peroxidase activity and total protein content due to treatments with 8-HQC or chlorine were not identified.
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