Bovine monoclonal antibodies specific for bovine herpesvirus-1 glycoprotein gIII

Srikumaran, S.; Onisk, D.V.; Zamb, T.J.; Osorio, F.A.; Rice, D.N.

Bovine monoclonal antibodies specific for bovine herpesvirus-1 glycoprotein gIII

1990

Srikumaran, S. | Onisk, D.V. | Zamb, T.J. | Osorio, F.A. | Rice, D.N.

Spleen cells from a calf immunized with bovine herpesvirus-1 (BHV-1) were fused with the nonsecreting murine cell line SP2/0. Several bovine-murine hybridomas secreting bovine immunoglobulins were stabilized. Of these, 9 hybridomas secreted bovine monoclonal antibodies that specifically bound to BHV-1 in a radioimmunoassay. Two of these monoclonal antibodies reacted specifically with BHV-1 in an indirect fluorescent antibody test and immunoprecipitated a BHV-1 glycoprotein with molecular mass of 97 kilodaltons.

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