One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose

Li, Siqiang; Wang, Hong; Ma, Juncai; Gu, Guofeng; Chen, Zonggang; Guo, Zhongwu

One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose

2016

A new, robust one-pot four-enzyme synthetic method was developed for thymidinediphosphate-l-rhamnose starting from d-glucose-1-phosphate. The enzymes, Glc-1-P thymidylyltransferase, dTDP-Glc-4,6-dehydratase, dTDP-4-keto-6-deoxy-Glc-3,5-epimerase and dTDP-4-keto-Rha reductase were derived from Streptococcus pneumonia serotype 23F, expressed in Escherichia coli, and studied in detail to provide the first direct evidence for their functions.

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