Rapid Chromatographic Immunoassay Study of Brain PrPˢᶜ Distribution in Classical Scrapie
2010
Bolea, Rosa | Vidal, Enric | Belén, Marín | Mercedes, Márquez | Vargas, Antonia | Marté, Pumarola | Juan, José Badiola
In the current study, a rapid chromatographic immunoassay submitted for registration in Europe was used to analyze PrPˢᶜ in 13 different areas of brain from 10 confirmed classical scrapie cases. The levels of PrPˢᶜ in the different areas of brain were plotted to draw a brain PrPˢᶜ distribution curve. This curve was compared with the brain PrPˢᶜ distribution curve obtained from immunoblotting and immunohistochemistry tests on the same samples. The distribution of PrPˢᶜ in different areas of the brain was similar, irrespective of the test applied, indicating that any of the 3 tests could be used for the characterization of classical cases of scrapie.
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