Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions
2022
Sandhu, Nitika | Singh, Jasneet | Singh, Gursewak | Sethi, Mehak | Singh, Mohini Prabha | Pruthi, Gomsie | Raigar, Om Prakash | Rupinder Kaur, | Kaur, Rupinder | Sarao, Preetinder Singh | Lore, Jagjeet Singh | Singh, Uma Maheshwar | Dixit, Shilpi | Sagare, Deepti B. | Singh, S.P. | Satturu, Vanisri | Singh, Vikas Kumar | Kumar, Arvind
The development and utilization of molecular-markers play an important role in genomics-assisted breeding during pyramiding of valuable genes. The aim of present study was to develop and validate a novel core-set of KASP (Kompetitive Allele-Specific PCR) markers associated with traits improving rice grain yield and adaptability under direct-seeded cultivation conditions. The 110 phenotypically validated KASP assays out of 171 designed KASP, include assays for biotic-resistance genes, anaerobic germination, root-traits, grain yield, lodging resistance and early-uniform emergence. The KASP assays were validated for their robustness and reliability at five different levels using diverse germplasm, segregating and advanced population, comparison with SSR markers and on F₁ₛ. The present research work will provide (i) breeding material in form of anticipated pre-direct-seeded adapted rice varieties (ii) single improved breeding line with many useful genes and (iii) KASP assay information for the useful QTL/genes providing grain yield and adaptability to rice under direct-seeded cultivation conditions.
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