Pseudomonas graminis as a biocontrol agent of fire blight
2011
Mikicinski, A. | Sobiczewski, P. | Jakubowska, A. | Pulawska, J. | Berczynski, S.
The goal of our study was to select bacterial isolates effective for control of fire blight and to run preliminary tests to elucidate their mechanism of action. Protective sprays of ‘Idared’ apple flowers on trees growing in the greenhouse with Pseudomonas graminis 49M strain at 2 concentrations (107 or 108 cfu/ml) showed that its efficacy against blossom blight was greater if a higher cell concentration was applied (89.0 versus 71.2% determined 5 days after inoculation with E. amylovora, strain Ea659). Strain 49M showed similar control efficacy to Pseudomonas fluorescens strain A506 and BlightBan A506 against blossom blight and Miedzian 50 WP (copper oxichloride) and Aliette 80 WG (fosetyl-Al) appeared to be less effective than 49M. Efficacy of 49M to reduce severity of fire blight of shoots of M.26 apple rootstock 7 and 17 days after inoculation with Ea659 ranged from 99.2 to 86.3%, respectively. Study on the biotic relationship between 49M and Ea659 on 3 artificial media (NAS, King’s B, LB) indicated that 49M inhibited growth of the pathogen on King’s medium B only. Strain 49M produced siderophores and biofilms, but not N-acyl homoserine lactones (AHL).
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