Interference of ferric ions with ferrous iron quantification using the ferrozine assay

Im, Jeongdae; Lee, Jaejin; Loeffler, Frank E.

Interference of ferric ions with ferrous iron quantification using the ferrozine assay

2013

Im, Jeongdae | Lee, Jaejin | Loeffler, Frank E.

The ferrozine assay is a widely used colorimetric method for determining soluble iron concentrations. We provide evidence for a heretofore unrecognized interference of ferric ions (Fe³⁺) on ferrous iron (Fe²⁺) measurements performed in the dark. Fe³⁺ concentrations affected the absorbance measurements, which linearly increased with incubation time.

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