Free amino acid concentrations and nitrogen isotope signatures in Pinus massoniana (Lamb.) needles of different ages for indicating atmospheric nitrogen deposition
2017
Xu, Yu | Xiao, Huayun
Free amino acid concentrations and nitrogen (N) isotopic composition in new current-year (new), mature current-year (middle-aged) and previous-year (old) Masson pine (Pinus massoniana Lamb.) needles were determined to indicate atmospheric N deposition in Guiyang (SW China). In different areas, free amino acids (especially arginine) concentrations in new and middle-aged needles were higher than in old needles, and the variation of free amino acids (especially arginine) concentrations in new and middle-aged needles was also greater than in old needles. This indicate that free amino acids in new and middle-aged needles may be more sensitive to N deposition compared to old needles. Moreover, concentrations of total free amino acids, arginine, histidine, γ-aminobutyric acid and alanine in middle-aged needles exhibited a strong relationship with N deposition (P < 0.05). Needle δ¹⁵N values showed a strong gradient from central Guiyang to the rural area, with more positive δ¹⁵N (especially in old needles) in the city center (0–5 km) and more negative δ¹⁵N (especially in old needles) in rural area (30–35 km). These suggest that N deposition in the urban center may be dominated by ¹⁵N-enriched NOx-N from traffic exhausts, while it is dominated by isotopically light atmospheric NHx-N from agriculture in rural area. Soil δ¹⁵N decreased slightly with distance from the city center, and the difference in δ¹⁵N values between the soil and needles (especially for old needles) increased significantly with the distance gradient, indicating that atmospheric N deposition may be an important N source for needles. This study provides novel evidence that free amino acids in needles and age-dependent needle δ¹⁵N values are useful indicators of atmospheric N deposition.
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