Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa
2008
Andrabi, S.M.H. | Ansari, M.S. | Ullah, N. | Anwar, M. | Mehmood, A. | Akhter, S.
We investigated the use of duck egg yolk (DEY), Guinea fowl egg yolk (GFEY) and Indian indigenous hen (Desi) egg yolk (IDEY) in extender for improving the post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa, and compared it with commercial hen egg yolk (CHEY; control). For this purpose, two consecutive ejaculates of semen from each of two Nili-Ravi buffalo bulls were collected on 1 day each week for 5 weeks (replicates; n =5) with artificial vagina (42°C). Split pooled ejaculates, were diluted in tris-citric acid glycerol extender containing either DEY or GFEY or IDEY or CHEY at 37°C. Extended semen was cooled to 4°C in 2h and equilibrated for 4h at 4°C. Cooled semen was then filled in 0.5ml straws at 4°C and frozen in programmable cell freezer. Thawing of semen was performed at 37°C for 30s. Sperm motility, plasma membrane integrity and sperm morphology (acrosome integrity, head, mid-piece and tail abnormalities) of each semen sample were assessed at 0, 3 and 6h after thawing and incubation at 37°C. Visual motility (%) and percentage of intact plasma membranes assessed at 6h post-thaw of buffalo bull spermatozoa were highest (P <0.05) due to DEY as compared to GFEY, IDEY and control. The percentage of spermatozoa with normal acrosomes at 0, 3 and 6h post-thaw was highest (P <0.05) in DEY extender than GFEY, IDEY and CHEY. Sperm tail abnormalities (%) observed at 0, 3 and 6h post-thaw in samples cryopreserved with freezing extender having DEY were lower (P <0.05) as compared to extender containing GFEY, IDEY and CHEY. In conclusion, DEY compared to other avian yolks in extender improves the frozen-thawed quality of buffalo bull spermatozoa.
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