Ameliorative effects of Lanthanum(Ⅲ) on Copper(Ⅱ) stressed rice (Oryza sativa) and its molecular mechanism revealed by transcriptome profiling
2020
Zhong, Yuqing | Chen, Jiajia
Rare earth elements are known to alleviate heavy metal stress. However, the potential mechanisms of the alleviation remain unclear. This study compared the effects of La(NO₃)₃ and La(NO₃)₃-amino acid chelates (La (Ⅲ)-AA) on growth, oxidative stress, ultrastructure, bioaccumulation and gene expression in rice. Results demonstrated that 20 mg/L La (Ⅲ)-AA can effectively ameliorate CuSO₄ (50 mg/L) stress in rice by reducing oxidative stress and increasing chlorophyll content, thus promoting growth. ICP and TEM revealed an antagonistic effect between La (Ⅲ) and Cu(Ⅱ). Exogenous La (Ⅲ)-AA decreased Cu(Ⅱ) content in rice leaves, stems and roots by 55.56%, 59.46% and 26.29%, and ameliorated Cu(Ⅱ) damage by maintaining the ultrastructure of mesophyll cells. RNA sequencing identified 7020 differentially expressed genes, and 8 were validated by qRT-PCR. Indole-3-acetic acid (IAA) concentration was detected by HPLC. Correlation analysis between OsGH3.4-IAA-Expansin revealed that IAA content is negatively correlated with OsGH3.4 (r = −0.82, P < 0.05), and positively correlated with Expansin (r = 0.78, P < 0.05). It's assumed that La (Ⅲ)-induced OsGH3.4 could inhibit IAA-dependent Expansin expression, thereby conferring resistance to Cu stress. This work provides novel insights into the molecular basis underlying La (Ⅲ)-induced Cu(Ⅱ) tolerance in rice.
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