Peptidomics of the pars intercerebralis–corpus cardiacum complex of the migratory locust, Locusta migratoria
2001
Clynen, Elke | Baggerman, Geert | Veelaert, Dirk | Cerstiaens, Anja | Horst, Dick van der | Harthoorn, Lucien | Derua, Rita | Waelkens, Etienne | De Loof, Arnold | Schoofs, Liliane
The pars intercerebralis–corpora cardiaca system (PI–CC) of insects is the endocrinological equivalent of the hypothalamus–pituitary system of vertebrates. Peptide profiles of the pars intercerebralis and the corpora cardiaca were characterized using simple sampling protocols in combination with MALDIâTOF and electrospray ionization double quadrupole time of flight (ESIâQqâTOF) mass spectrometric technologies. The results were compared with earlier results of conventional sequencing methods and immunocytochemical methods. In addition to many known peptides, several m/z signals corresponding to putative novel peptides were observed in the corpora cardiaca and/or pars intercerebralis. Furthermore, for a number of peptides evidence was provided about their localization and MALDIâTOF analysis of the released material from the corpora cardiaca yielded information on the hormonal status of particular brain peptides.
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