Yamadazyma siamensis sp. nov., Yamadazyma phyllophila sp. nov. and Yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in Thailand and Taiwan
2013
Kaewwichian, Rungluk | Yongmanitchai, Wichien | Kawasaki, Hiroko | Wang, Pi-Han | Yang, Shanhua | Limtong, Savitree
Four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (DMKU-RK254ᵀ), corn leaves (DMKU-RK548ᵀ), bean leaves (K129) in Thailand and hengchun pencilwood leaves (TrB1-1ᵀ) in Taiwan. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region, the actin gene (ACT1) and the elongation factor 2 gene (EF2), the four strains were determined to represent novel Yamadazyma species although formation of ascospores was not observed. Strain DMKU-RK254ᵀ was determined to be related to Candida diddensiae, Candida naeodendra and Candida kanchanaburiensis but with 1.8, 1.8 and 2.0 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, respectively. It was assigned to Yamadazyma siamensis sp. nov. (type strain DMKU-RK254ᵀ = BCC 50730ᵀ = NBRC 108901ᵀ = CBS 12573ᵀ). The sequences of the D1/D2 region of the LSU rRNA gene, the ITS region, ACT1 gene and EF2 gene of two strains (DMKU-RK548ᵀ and K129) were identical but differed from that of strain TrB1-1ᵀ by 0.6, 1.0, 3.3 and 5.9 % nucleotide substitutions, respectively. Therefore, the two strains (DMKU-RK548ᵀ and K129) and strain TrB1-1ᵀ were assigned to be two separate species. The closest species in terms of pairwise sequences similarity of the D1/D2 region to the two novel species was Yamadazyma philogaea but with 1.1–1.7 % nucleotide substitutions. The two strains (DMKU-RK548ᵀ and K129) were assigned to Yamadazyma phyllophila sp. nov. (type strain DMKU-RK548ᵀ = BCC 50736ᵀ = NBRC 108906ᵀ = CBS 12572ᵀ) and the strain TrB1-1ᵀ was named Yamadazyma paraphyllophila sp. nov. (type strain TrB1-1ᵀ = BCRC 23030ᵀ = CCTCC AY 204005ᵀ = CBS 9928ᵀ).
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