Sphingobacterium rhinopitheci sp. Nov., isolated from the faeces of Rhinopithecus bieti in China
2021
Han, Xiu-Lin | Wang, Qiong | Zhang, Chen-Lu | Fang, Zhi-Qin | Shen, Jian-Qiang | Huang, Song | Lu, Tao
A novel bacterium, WQ 047ᵀ, was isolated from the faeces of Rhinopithecus bieti, a highly endangered primate endemic to China. The cells were aerobic, oval/rod-shaped, Gram-stain-negative, non-motile, catalase positive, and produced yellow pigmented colonies on Columbia Agar. The taxonomic position of WQ 047ᵀ was clarified by applying a polyphasic study based on 16S rRNA gene sequence phylogenetic analysis, extensive biological typing, and whole genome sequencing. Phylogenetic analysis indicated that stain WQ 047ᵀ belonged to the genus Sphingobacterium and its 16S rRNA gene sequence exhibited 96.47% pairwise similarity with that of the closest relatives Sphingobacterium nematocida M-SX103ᵀ. The calculated whole genome average nucleotide identity (ANI) value between strain WQ 047ᵀ and strain M-SX103 was 72.3%. The digital DNA-DNA hybridization value of strain WQ 047ᵀ and M-SX103ᵀ was 15.73%, which was obtained by calculating the genome-to-genome distance. The major fatty acids were C₁₅:₀ iso, C₁₇:₀ iso 3-OH, Summed Feature 3 (C₁₆:₁ ω7c/C₁₆:₁ ω6c) and Summed feature 9 (iso-C₁₇:₁ω9c and/or 10-methyl C₁₆:₀). The predominant polar lipids were PE, PL and APL. MK-7 was the predominant menaquinone. The G + C content of WQ 047ᵀ was 34.89 mol% according to genome analysis. All these characteristics were consistent with those of the genus of Sphingobacterium. Therefore, based on these results, we propose a novel species for which the name Sphingobacterium rhinopitheci sp. Nov. is proposed, with the type strain WQ 047ᵀ (= CCTCC AA 2020026ᵀ = KCTC82393ᵀ).
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