Identification of molecular markers linked to adult plant leaf rust resistance gene Lr48 in wheat and detection of Lr48 in the Thatcher near-isogenic line with gene Lr25
2010
Samsampour, D | Maleki Zanjani, B | Pallavi, J. K | Singh, Anupam | Charpe, A | Gupta, S. K | Prabhu, K. V
The recessive adult plant resistance (APR) gene Lr48 in wheat was tagged with flanking random amplified polymorphic DNA (RAPD) markers. Markers S336₇₇₅ in coupling and S3₄₅₀ in repulsion with Lr48 were identified in wheat line CSP44. Tests of these markers on available Thatcher near-isogenic lines (NILs) detected the likely presence of Lr48 in TcLr25. A test of allelism of APR involving the cross TcLr25 × CSP44 indicated that Lr48 was present in both lines. A separate experiment on inheritance of resistance in an F₂ population of TcLr25 × Agra Local confirmed the presence of a dominant seedling resistance gene (Lr25) and a recessive APR gene (Lr48) in TcLr25. This study demonstrated the value of molecular markers in identifying the presence of masked genes in genetic stocks where direct phenotyping failed to detect their presence.
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