Functioning of the TA cassette of streptococcal plasmid pSM19035 in various Gram-positive bacteria
2012
Brzozowska, Iwona | Brzozowska, Kinga | Zielenkiewicz, Urszula
Toxin–antitoxin (TA) systems are common in microorganisms and are frequently found in the chromosomes and low-copy number plasmids of bacterial pathogens. One such system is carried by the low copy number plasmid pSM19035 of the pathogenic bacterium Streptococcus pyogenes. This plasmid encodes an omega-epsilon-zeta cassette that ensures its stable maintenance by post-segregational killing of plasmid-free cells. In this study, the activity of the ω-ε-ζ cassette was examined in various Gram-positive bacteria with a low G/C content in their DNA. The broad host range of pSM19035 was confirmed and the copy number of a truncated derivative in transformed strains was determined by real-time qPCR.
显示更多 [+] 显示较少 [-]