Orrella daihaiensis sp. nov., a bacterium isolated from Daihai Lake in Inner Mongolia
2022
Jiang, Kai | Yuan, Bo | Cao, ChunLing | Zhang, Chenying | Liu, Yang | Hai, XiaoHu | Li, RuoXuan | Qian, KangYuan | Yang, HongZhen
A novel aerobic, Gram-staining-negative, non-motile, short-rod-shaped strain, designated f23ᵀ, was obtained from Daihai Lake, Inner Mongolia, Republic of China. 16S rRNA gene sequences analysis showed that f23ᵀ belongs to the genus Orrella and is most closely related to Orrella marina H-Z20ᵀ with 98.35% sequence similarity. The strain was oxidase positive, catalase positive and had well growth at pH 6.5–8.5, at temperature 28–40 °C and at 0–4.5% (w/v) NaCl. Colonies incubated at 37 °C on marine 2216 agar for 3 days were white, smooth, transparent, circular and less than 1.0 mm in diameter. The total genome size of f23ᵀ was 2,803,849 bp with a G + C content of 52.79%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain f23ᵀ and O. marina H-Z20ᵀ were 69.62% and 20.5%, which both below the species delineation threshold. Chemotaxonomic analysis showed that C₁₆:₀, cyclo-C₁₇:₀, C₁₈:₀, Sum Feature 3 (C₁₆:₁ω7c and/or C₁₆:₁ω6c) and Sum Feature 8 (C₁₈:₁ω6c and C₁₈:₁ω7c) as the major fatty acids, ubiquinone-8 as the major isoprenoid quinone, phosphatidylethanolamine as the major cellular polar lipids. Based on the polyphasic analysis, f23ᵀ represents a novel species within the genus Orrella, for which the name Orrella daihaiensis sp. nov. is proposed. The type strain is f23ᵀ (= CGMCC 1.18761 ᵀ = KCTC 82425 ᵀ).
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