Tratamiento de semilla de chile con acidos salicilico y sulfosalicilico y respuesta de las plantulas al frio | Pepper seed treatment with salicylic and sulfosalicylic acid and response of seedlings to cold stress
2004
Benavides-Mendoza, A. | Salazar-Torres, A.M. | Ramirez-Godina, F. | Robledo-Torres, V. | Ramirez-Rodriguez, H. | Maiti, R.
It is known that salicylic acid (AS) is a stress signaling compound in plants. With the objective of verifying if the exogenous application of AS and a sulfur derivative induces resistance to cold stress, we applied a six hours pretreatment of pepper seeds with a water solution of AS and sulfosalicylic acid (ASS) at concentrations of 0, 10(-5), 10(-4), and 10(-3) M in a controlled temperature chamber. After germination, the seeds were transferred to containers with sphagnum peat moss with Douglas nutrient solution; 22 days after transplanting the seedlings were twice exposed to cold stress in 24 h periods in a refrigerator at 4 degrees C without light. There were four days between the first and second cold events. Seed application of AS 10(-4) M and ASS 10(-4) M was effective in inducing seedling resistance to cold stress, manifested as taller seedlings and more plant fresh and dry weight. AS and ASS 10(-3) M and 10(-5) M showed a non significant effect on seedling weight. Stomatal index and stomatal density were negatively affected by AS and ASS 10(-3) M. On the other hand AS and ASS 10(-4) and 10(-5) M increased the stomatal index and stomatal density in abaxial side, showing the opposite response in the adaxial side. Stomatal density in abaxial side showed a significant correlation with plant weight, leaf number, and plant height.
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