Respiratory characteristics of isolated barley mitochondria and intact barley roots
1993
McDonnell, E. | Farrar, J.F.
Purified mitochondria were prepared from roots of 7- and 14-d-old barley plants and their respiratory activities were determined. The following observations were made. (1) With age, total oxygen uptake declined in the intact root and in isolated root mitochondria. (2) The alternative pathway was present and engaged to 60% of its capacity in the intact roots of both 7- and 14-d-old plants. In the isolated mitochondria its activity depended on the substrate used. (3) State 4 respiration rates were high in the isolated mitochondria, but there was little contribution by the alternative oxidase. The implications of these findings are discussed. It is proposed that mitochondrial respiratory capacity plays an important role in determining the respiration rate of the intact barley root.
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