Sources of error in dietary fibre analysis
1994
Manas, E. | Bravo, L. | Saura-Calixto, F.
The following error sources in the present dietary fiber (DF) analytical methods were investigated: (1) the omission of the protease treatment of samples may modify the results by increasing the Klason lignin fraction and altering the content and/or distribution of polysaccharides; (2) some soluble dietary fibre (SDF) constituents can be retained in the insoluble dietary fibre (IDF) matrix affecting the insoluble and soluble fraction distribution; (3) protein, ash and blank corrections in gravimetric analysis involve a lack of precision, over- or undervaluing the actual DF contents; (4) the Klason lignin fractions obtained by acid hydrolysis of DF residues are made up of different components and artifacts besides lignin. These studies included both new observations and additional quantitative evidence on error sources previously mentioned in the literature. In some cases the published methods were modified to emphasize the methodological errors.
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