Ultrasound assisted microextraction-nano material solid phase dispersion for extraction and determination of thymol and carvacrol in pharmaceutical samples: Experimental design methodology
2015
Roosta, Mostafa | Ghaedi, Mehrorang | Daneshfar, Ali | Sahraei, Reza
In the present study, for the first time, a new extraction method based on “ultrasound assisted microextraction-nanomaterial solid phase dispersion (UAME-NMSPD)” was developed to preconcentrate the low quantity of thymol and carvacrol in pharmaceutical samples prior to their HPLC-UV separation/determination. The analytes were accumulated on nickel sulfide nanomaterial loaded on activated carbon (NiS-NP-AC) that with more detail identified by XRD, FESEM and UV–vis technique. Central composite design (CCD) combined with desirability function (DF) was used to search for optimum operational conditions. Working under optimum conditions specified as: 10min ultrasonic time, pH 3, 0.011g of adsorbent and 600μL extraction solvent) permit achievement of high and reasonable linear range over 0.005–2.0μgmL−1 (r2>0.9993) with LOD of thymol and carvacrol as 0.23 and 0.21μgL−1, respectively. The relative standard deviations (RSDs) were less than 4.93% (n=3).
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