Structural Characterization and Property Analysis of Surface-Active Substance Accumulated During Biodegradation of Hydrophobic α-Pinene by Pseudomonas fluorescens
2013
Cheng, Zhuo-Wei | Chen, Jian-Meng | Yu, Jian-ming | Li, Shan-Shan | Jiang, Yi-Feng
The connection between hydrocarbon biodegradation and surface-active substance production has attracted great interest in recent years. Pseudomonas fluorescens PT, isolated from a biotrickling filter, was not only able to degrade α-pinene but also to use it as a carbon source to produce a surface-active substance. Response surface methodology analysis showed that the optimal medium composition was K⁺, 69.8; Mn²⁺, 65.1; and NH₄ ⁺, 482.5 mg L⁻¹, at which the surface tension of the medium was reduced to 40.7 mN m⁻¹ after 36 h. Based on compositional analysis and information on α-pinene metabolism, the purified compound was identified as perilla acid. The surface-active properties of the purified compound were more stable than those of a synthetic surfactant, and it had lower ecological toxicity to Chlorella vulgaris. The naphthalene solubility and mass transfer of α-pinene were enhanced almost twofold by the surface-active substance (at its critical micelle concentration). The results suggested that the PT strain may be promising for generating surface-active substances with improved physiochemical properties for a wide range of applications in environmental remediation.
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